TB-500

99%+ Purity USA Tested

Research Use Only. Not for Human or Veterinary Use

TB-500 (Thymosin Beta-4): Actin-sequestering cell migration peptide, CAS 77591-33-4, MW 4963.50 g/mol, ≥99% purity. Lyophilized powder for laboratory use.

$65.00

Peptide Name	TB-500 (Thymosin Beta-4 Fragment)
CAS Number	77591-33-4
Molecular Formula	C₂₁₂H₃₅₀N₅₆O₇₈S
Molecular Weight	4963.50 g/mol
Mechanism Class	Actin-sequestering and cell migration peptide
Appearance	White lyophilized powder
Purity	≥99% (HPLC)
Storage	-20°C, desiccated, protected from light
Solubility	Soluble in sterile water

This product is not intended for human or animal use. For scientific research only.

VivePeptides provides detailed analytical results including High-Performance Liquid Chromatography (HPLC) and mass spectrometry data for all peptides. These reports confirm peptide purity, composition, and molecular accuracy, giving researchers full transparency and confidence in the materials used for their studies.

VIEW COA

VivePeptides peptides should be stored in a cool, dry environment and protected from light and moisture. Lyophilized peptides are best kept refrigerated or frozen until use to maintain stability. Once reconstituted for research purposes, peptides should be stored according to standard laboratory protocols, typically under refrigeration, and handled using proper aseptic techniques to preserve integrity throughout the study period.

OVERVIEW

What Is TB-500 Peptide?

TB-500 is a synthetic peptide based on the active region of Thymosin Beta-4 (Tβ4), a highly conserved 43-amino-acid protein first isolated from the thymus gland. Tβ4 is one of the most abundant intracellular peptides in mammalian tissues and plays a central role in actin dynamics, cell motility, and tissue repair signaling. TB-500 encompasses the key functional domain responsible for actin sequestration and has been the subject of extensive preclinical research over the past two decades.

As a research peptide, TB-500 is primarily studied for its role in regulating the actin cytoskeleton and promoting cell migration. Research suggests that TB-500 interacts with G-actin monomers, preventing premature polymerization and maintaining a pool of monomeric actin available for directed cell movement. This mechanism, combined with TB-500’s observed effects on angiogenesis and extracellular matrix remodeling in laboratory settings, has made it a foundational compound in tissue repair and regeneration research worldwide.

Actin-Binding Peptide

Synthetic fragment of Tβ4 containing the key LKKTETQ actin-sequestration domain

≥99% HPLC Purity

Every batch verified via high-performance liquid chromatography

USA Tested & Verified

Third-party analytical testing performed in USA laboratories

RESEARCH

TB-500 Mechanism of Action in Research

The TB-500 mechanism of action centers on its interaction with the actin cytoskeleton and its downstream effects on cell migration, angiogenesis, and tissue remodeling. Published research has identified multiple pathways through which TB-500 influences cellular behavior in laboratory settings. TB-500 research continues to expand our understanding of how Thymosin Beta-4-derived peptides regulate tissue repair processes.

Actin Sequestration and Cytoskeletal Regulation

TB-500 binds to G-actin (globular actin) monomers through its LKKTETQ domain, preventing spontaneous F-actin (filamentous actin) polymerization. Research by Safer et al. (1997) demonstrated that Tβ4, and by extension its active fragment TB-500, functions as a major actin-sequestering protein, maintaining the dynamic equilibrium between G-actin and F-actin pools within cells. This regulation of actin dynamics is essential for directed cell migration, lamellipodia formation, and cellular remodeling processes studied in tissue repair research.

Cell Migration and Angiogenesis Promotion

Studies indicate that TB-500 promotes both cell migration and angiogenesis through complementary mechanisms. Malinda et al. (1999) published findings in the Journal of Investigative Dermatology demonstrating that Tβ4 promoted endothelial cell migration and tube formation — hallmarks of angiogenesis — in vitro. Additional research by Philp et al. (2004) showed that Tβ4-derived peptides increased matrix metalloproteinase (MMP) expression and enhanced extracellular matrix remodeling, providing mechanistic insight into how TB-500 facilitates tissue reorganization in research models.

Anti-Inflammatory and Survival Signaling

Beyond cytoskeletal effects, TB-500 research has revealed anti-inflammatory and cell survival properties. Sosne et al. (2007) published studies demonstrating that Tβ4 downregulated NF-κB-mediated inflammatory signaling and modulated cytokine expression in tissue injury models. Research also suggests that TB-500 may activate Akt-mediated survival pathways, contributing to reduced apoptosis in stressed cellular environments, adding another dimension to the peptide’s tissue-protective research profile. —

COMPARISON

TB-500 vs BPC-157: Research Peptide Comparison

TB-500 and BPC-157 are two of the most frequently studied peptides in tissue repair and regeneration research. While they share overlapping research applications, they differ significantly in origin, structure, and proposed mechanisms of action. Understanding these differences helps researchers select the appropriate compound for their specific laboratory protocols.

Feature	TB-500 (Thymosin Beta-4)	BPC-157
Origin	Fragment of Thymosin Beta-4 (thymus gland)	Derived from human gastric juice protein
Amino Acids	43	15
CAS Number	77591-33-4	137525-51-0
Molecular Weight	4963.50 Da	1419.53 Da
Primary Research Focus	Cell migration and tissue repair	Gastric and tissue research
Key Mechanism	Actin sequestration, cell motility	NO system, growth factor modulation
Angiogenesis	Promotes via endothelial cell migration	Promotes via VEGF pathways
Anti-Inflammatory	NF-κB modulation	Cytokine modulation
Research Volume	50+ published studies	100+ published studies

Both peptides are valuable tools in tissue repair research, each acting through distinct molecular mechanisms. Some researchers have explored their combined use in preclinical models, though the majority of published literature examines each compound independently. VivePeptides offers both TB-500 for sale and BPC-157 at research grade purity from our USA-based facility.

RESEARCH STUDIES

TB-500 Research Applications & Published Studies

TB-500 research spans two decades of published preclinical literature focused on tissue repair, cell migration, and regenerative biology. The following areas represent the most actively investigated applications of TB-500 peptide. All references are to research contexts only.

Wound Healing and Tissue Repair Research

The primary body of TB-500 research concerns tissue repair mechanisms. Malinda et al. (1999) published foundational work in the Journal of Investigative Dermatology demonstrating that Thymosin Beta-4 accelerated dermal wound closure in animal models. Philp et al. (2004) expanded this research in the FASEB Journal, showing that Tβ4 promoted full-thickness dermal wound repair through enhanced cell migration, angiogenesis, and collagen deposition. These studies established TB-500 as a key research compound in wound biology.

Cardiac Tissue Research

Research has explored TB-500 in cardiac tissue models with significant findings. Bock-Marquette et al. (2004) published a landmark study in Nature demonstrating that Tβ4 promoted survival of cardiac myocytes and enhanced cardiac function in murine models following injury. Smart et al. (2007) further demonstrated that Tβ4 could reactivate epicardial progenitor cells, opening new avenues of investigation in cardiac regeneration research.

Corneal and Ocular Research

TB-500 research has yielded notable results in ocular tissue models. Sosne et al. (2002) published studies demonstrating that Tβ4 promoted corneal wound healing and reduced inflammatory markers in ocular surface injury models. Subsequent research by the same group (Sosne et al., 2007) investigated Tβ4’s anti-inflammatory mechanisms in corneal epithelial cells, documenting NF-κB pathway modulation and cytokine regulation.

QUALITY ASSURANCE

Quality & Testing Standards

VivePeptides maintains rigorous quality control for every batch of TB-500 peptide. Our commitment to research-grade purity ensures that laboratories receive consistent, reliable compounds for their investigations.

HPLC & Mass Spectrometry

Every batch of TB-500 undergoes high-performance liquid chromatography (HPLC) and mass spectrometry analysis to confirm identity, purity, and molecular weight.

Third-Party Verified

All VivePeptides TB-500 is independently verified through third-party analytical laboratories based in the USA. Testing documentation is available for every lot.

≥99% Purity Standard

Our TB-500 consistently meets or exceeds ≥99% purity as determined by HPLC analysis, ensuring research-grade quality for laboratory applications.

FAQ

Frequently Asked Questions About TB-500

What is TB-500 used for in research?

TB-500 is used in preclinical research to study actin dynamics, cell migration, angiogenesis, and tissue repair mechanisms. As a synthetic fragment of Thymosin Beta-4, it is a key research tool for investigating cytoskeletal regulation and regenerative biology. TB-500 is sold for research use only.

What purity is VivePeptides TB-500?

VivePeptides TB-500 is manufactured to ≥99% purity as verified by HPLC analysis. Every batch is tested and verified through independent third-party USA-based laboratories to ensure consistent research-grade quality.

How should TB-500 be stored?

Lyophilized TB-500 should be stored at -20°C, protected from light and moisture. Once reconstituted, the solution should be stored at 2–8°C and used within 14 days for optimal stability. Avoid repeated freeze-thaw cycles.

What is the difference between TB-500 and Thymosin Beta-4?

TB-500 is commonly used as a synonym for synthetic Thymosin Beta-4 (Tβ4) in the research peptide market. Both refer to the full 43-amino-acid sequence of Tβ4 or its active fragment. The LKKTETQ domain within the sequence is the key actin-binding region responsible for the peptide’s biological activity in research models.

Is VivePeptides TB-500 third-party tested?

Yes. Every lot of VivePeptides TB-500 undergoes independent third-party testing at USA-based analytical laboratories. Testing includes HPLC purity analysis and mass spectrometry identity confirmation. Results are available upon request for qualified researchers.

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